Insertion junction: CLIP2.033240_1

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:+
Strain:CLIP2.033240
Chromosome:chromosome 2
Location:4174956
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre02.g103950 MIA1,FAP100 (1 of 2) PF13863 - Domain of unknown function (DUF4200) (DUF4200); MIA1 subunit of the modifier of inner arms (MIA) complex CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACAAGAAGATTTCGCGCTTCCTGCATGGACAGGGCGTGCGGGGTGAGACA
Internal bar code:TTCGTGATGACGGGCTTAGCCA

Confirmation - LEAP-Seq

LEAP-Seq distance:2201
LEAP-Seq percent confirming:95.1219
LEAP-Seq n confirming:39
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:41

Suggested primers for confirmation by PCR

Suggested primer 1:CTCAACCGCTGCCATAATGC
Suggested primer 2:AAAACACGTTACGGCAGTGC