| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.033251 |
| Chromosome: | chromosome 1 |
| Location: | 4032466 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g026600 | UBC11 | (1 of 1) K10580 - ubiquitin-conjugating enzyme E2 N (UBE2N, BLU, UBC13); E2 Ubiquitin conjugating enzyme | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGCTTGTCGATGCTGCGAAGGCAAGGCAAGGCGCCAAATCAAACCCCAGC |
| Internal bar code: | ACTTATTTCGTCTGGTGTGAAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2945 |
| LEAP-Seq percent confirming: | 87.5 |
| LEAP-Seq n confirming: | 7 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGCAACATATGTGAGGCGC |
| Suggested primer 2: | GGGTTTGGGATGGCTTAGCT |