| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.033261 |
| Chromosome: | chromosome 3 |
| Location: | 3294114 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g165750 | (1 of 1) K03018 - DNA-directed RNA polymerase III subunit RPC1 (RPC1, POLR3A) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCACACATGCATATGTGTGGTTGCAGGTGGGAGCCCTGTTCGAGCGGCC |
| Internal bar code: | GGGGGCCACAGGTTATTTATAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2369 |
| LEAP-Seq percent confirming: | 97.4359 |
| LEAP-Seq n confirming: | 38 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 39 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGACTCCTCCACTCCTCCC |
| Suggested primer 2: | ACACCAACACCAAACCCCAT |