| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | - |
| Strain: | CLIP2.033272 |
| Chromosome: | chromosome 1 |
| Location: | 4054272 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g800077 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGGCTGCGGACGACGCTGCTGACTCCAGCCGTGCGAGGTCATCTGCGCA |
| Internal bar code: | AGCGGAAGGTAATAGAGCGTAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1757 |
| LEAP-Seq percent confirming: | 93.75 |
| LEAP-Seq n confirming: | 15 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTCTGGTCCTGGACTTTGGG |
| Suggested primer 2: | TTGGATCGCTTGCAGGTAGG |