Insertion junction: CLIP2.033298_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:+
Strain:CLIP2.033298
Chromosome:chromosome 12
Location:5035515
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre12.g525400 PTK15 (1 of 55) PTHR23257//PTHR23257:SF394 - SERINE-THREONINE PROTEIN KINASE // SUBFAMILY NOT NAMED; Protein tyrosine kinase CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGTGGTGGTCTTCAACGGCAACCACGAGAGCCGCAAGGTGCGCGTGGCGG
Internal bar code:TTCAAACGCATGCATAGTTGGT

Confirmation - LEAP-Seq

LEAP-Seq distance:1465
LEAP-Seq percent confirming:90.0
LEAP-Seq n confirming:27
LEAP-Seq n nonconfirming:3
LEAP-Seq n unique pos:30

Suggested primers for confirmation by PCR

Suggested primer 1:AGCAGCTCCCGCTTAATGTT
Suggested primer 2:CAGTGAGGTGCAGCTGATGA