Insertion junction: CLIP2.033305_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.033305
Chromosome:chromosome 7
Location:488835
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre07.g315650 SMM23 (1 of 1) 2.1.1.164 - Demethylrebeccamycin-D-glucose O-methyltransferase; S-adenosyl-L-methionine-dependent methyltransferase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACTGGCGCGGCACGCAAACAAAGGATCCTCATCTGTGCCCCCTCACAACC
Internal bar code:CCGTTTATTAACTTTCGACATC

Confirmation - LEAP-Seq

LEAP-Seq distance:48
LEAP-Seq percent confirming:50.0
LEAP-Seq n confirming:1
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:CACGTACAACAAGCGCAACA
Suggested primer 2:CACCAGACAGCAACAGCAAC