| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.033314 |
| Chromosome: | chromosome 7 |
| Location: | 2722271 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g331150 | (1 of 2) 1.3.3.5 - Bilirubin oxidase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGGCGGCAGCAGCCCAAGCTGCATGTGCAAGCGACGGCAGCACTACTTG |
| Internal bar code: | TGAAACTCAAGCGATTAGCTCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2714 |
| LEAP-Seq percent confirming: | 92.3077 |
| LEAP-Seq n confirming: | 36 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 39 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGTCACAGGAGCCGATCAA |
| Suggested primer 2: | ATCTGTACGGACCGAGGTGA |