| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.033317 |
| Chromosome: | chromosome 12 |
| Location: | 236071 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g485000 | FAP182 | (1 of 2) PTHR20899:SF1 - UPF0691 PROTEIN C9ORF116; Flagellar Associated Protein 182 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACCCCGTCCGGACCCTCAATCAGGTGTGTATACGACCCTGTGACCCAAGG |
| Internal bar code: | GCGGGAGATTATCTTCTGACCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1772 |
| LEAP-Seq percent confirming: | 94.1176 |
| LEAP-Seq n confirming: | 16 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 17 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTCTTGTCAGCGATCCACCA |
| Suggested primer 2: | TAAGTACGTTGTCCAGCGGG |