Insertion junction: CLIP2.033333_2

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:+
Strain:CLIP2.033333
Chromosome:chromosome 3
Location:2833808
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre03.g162100 PIMT2 Protein-L-isoaspartate O-methyltransferase; (1 of 3) K00573 - protein-L-isoaspartate(D-aspartate) O-methyltransferase (E2.1.1.77, pcm) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTGCCTGGTCGTGTGTGACTCGAGTATGCAACTAAGCTGGGCCGGCGCGC
Internal bar code:ACTCACCTGACATCTGAATTAG

Confirmation - LEAP-Seq

LEAP-Seq distance:797
LEAP-Seq percent confirming:36.0
LEAP-Seq n confirming:9
LEAP-Seq n nonconfirming:16
LEAP-Seq n unique pos:25

Suggested primers for confirmation by PCR

Suggested primer 1:CTTCAAAGTTCACACCCGCG
Suggested primer 2:CCGAACATGCGCCTCTATCT