Insertion junction: CLIP2.033338_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.033338
Chromosome:chromosome 12
Location:4021287
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre12.g516150 NAT27 N-acetyltransferase; (1 of 16) 2.3.1.48 - Histone acetyltransferase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGCTGCCACGCCCTTGCCTGACCCTCGTGCCACCCCTGCGCCTGCCGCTC
Internal bar code:CTGCGGTCCAGAGACGAAGGAG

Confirmation - LEAP-Seq

LEAP-Seq distance:2801
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:51
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:51

Suggested primers for confirmation by PCR

Suggested primer 1:ACAAGCCCAAACCCTGATCG
Suggested primer 2:ACCTCCCCCTCCAAGCATAT