| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.033374 |
| Chromosome: | chromosome 3 |
| Location: | 5924283 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g188800 | Nicotinate phosphoribosyltransferase; (1 of 1) PTHR11098//PTHR11098:SF2 - NICOTINATE PHOSPHORIBOSYLTRANSFERASE // SUBFAMILY NOT NAMED | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CATGCTTCCTGTCGGTGTTCCCATAAGCGTCTTGACGGACAGTTACAAGG |
| Internal bar code: | GACTTAGGCAAGGCATATGCTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4071 |
| LEAP-Seq percent confirming: | 80.9524 |
| LEAP-Seq n confirming: | 34 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 42 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGGGTACAAAGGCGTCCTG |
| Suggested primer 2: | CCACGTCACTTGATCCCACA |