| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.033399 |
| Chromosome: | chromosome 7 |
| Location: | 6456527 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre40.g760147 | (1 of 1) PTHR10877//PTHR10891 - POLYCYSTIN-RELATED // EF-HAND CALCIUM-BINDING DOMAIN CONTAINING PROTEIN | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CACACACACACACACACACACACACACACACACACACACACACACACACA |
| Internal bar code: | AAATCGTCCGATAACTGGGATT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3224 |
| LEAP-Seq percent confirming: | 94.8276 |
| LEAP-Seq n confirming: | 55 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 58 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGACACACAGCAGCACAAC |
| Suggested primer 2: | CCACACACACACACACACAC |