Insertion junction: CLIP2.033402_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.033402
Chromosome:chromosome 12
Location:4081056
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre12.g801400 (1 of 31) PF03732 - Retrotransposon gag protein (Retrotrans_gag) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGGGGTTGAGAGCTCCATCACCACCTGGGCGCAGTTCCAAATCACTTTCC
Internal bar code:TCCTAGTGTCGCAGAAATAGGA

Confirmation - LEAP-Seq

LEAP-Seq distance:4086
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:40
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:40

Suggested primers for confirmation by PCR

Suggested primer 1:GTTGCACGTCATGATGGTCG
Suggested primer 2:GTCGGCTAGTCGCACGATTA