Insertion junction: CLIP2.033406_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.033406
Chromosome:chromosome 2
Location:2527223
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre02.g092250 FKB42,FKB22 Peptidyl-prolyl cis-trans isomerase, FKBP-type; (1 of 2) PF00254//PF07719//PF13414 - FKBP-type peptidyl-prolyl cis-trans isomerase (FKBP_C) // Tetratricopeptide repeat (TPR_2) // TPR repeat (TPR_11) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCTCAACATGGCCGCCACACAGCTCAAGACAGGGGACTGGAACACGGCCA
Internal bar code:GTGCACTGTGTAGGAGCTGGAT

Confirmation - LEAP-Seq

LEAP-Seq distance:1924
LEAP-Seq percent confirming:83.6066
LEAP-Seq n confirming:51
LEAP-Seq n nonconfirming:10
LEAP-Seq n unique pos:61

Suggested primers for confirmation by PCR

Suggested primer 1:TGGCCATGCTGGTCTTGTAG
Suggested primer 2:TGCCCTTTCCACGTCACTAC