| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.033414 |
| Chromosome: | chromosome 5 |
| Location: | 1332454 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g241655 | (1 of 24) IPR001478 - PDZ domain | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTGCCCCGGCCCAATGAAATAGGTCCCAGTGGGGTTACATTCATGATTA |
| Internal bar code: | CTGTCAATTTGTCTTTTCCTTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1215 |
| LEAP-Seq percent confirming: | 96.0784 |
| LEAP-Seq n confirming: | 49 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 51 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TACCGTTTCGTTTCGACCGT |
| Suggested primer 2: | ACAAGCGTCACGTCCATCTT |