Insertion junction: CLIP2.033429_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.033429
Chromosome:chromosome 10
Location:1964009
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre10.g432000 DDB1 (1 of 1) K10610 - DNA damage-binding protein 1 (DDB1); UV-damaged DNA binding complex subunit 1 protein intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTGGCCAACATGCGTGCACGCATTCCGTTACGTACTGTAATACAGGGCGG
Internal bar code:TCGTATTCTGTGTTTAGGCGCA

Confirmation - LEAP-Seq

LEAP-Seq distance:665
LEAP-Seq percent confirming:20.0
LEAP-Seq n confirming:3
LEAP-Seq n nonconfirming:12
LEAP-Seq n unique pos:15

Suggested primers for confirmation by PCR

Suggested primer 1:TCCTCCGACTCCGACTCTTT
Suggested primer 2:AATGCGATCGAGGACCATCC