| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.033446 |
| Chromosome: | chromosome 16 |
| Location: | 6642678 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g673600 | (1 of 19) K08850 - aurora kinase, other [EC:2.7.11.1] (AURKX) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATACTTCCGACAGCAAACCATTCGATCCAGTCTCTCACTTGTGCACGGCG |
| Internal bar code: | AAGTATAATTGCCGACCCGCGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1313 |
| LEAP-Seq percent confirming: | 82.1429 |
| LEAP-Seq n confirming: | 23 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 28 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTGTCTAACCATGTCCCCGC |
| Suggested primer 2: | TACACGCGCTCCTTTTGTCT |