| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.033455 |
| Chromosome: | chromosome 1 |
| Location: | 5696202 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| lncRNA_TCONS_00249475 |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCGAGCTGTACACTAACCTGATTTCTACGCGACACCGCTTTATTTCCCG |
| Internal bar code: | CCGGTATTATTTAGTGTGCCGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3508 |
| LEAP-Seq percent confirming: | 75.6757 |
| LEAP-Seq n confirming: | 56 |
| LEAP-Seq n nonconfirming: | 18 |
| LEAP-Seq n unique pos: | 74 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTGAGCTGCTCCTTCAGGTG |
| Suggested primer 2: | TGTTTGTGTGGTCACCAGCT |