| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | - |
| Strain: | CLIP2.033473 |
| Chromosome: | chromosome 7 |
| Location: | 331506 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g314400 | (1 of 1) PF00580//PF13538 - UvrD/REP helicase N-terminal domain (UvrD-helicase) // UvrD-like helicase C-terminal domain (UvrD_C_2) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGCATGCTCCAGGAAGCGGCCCACTCCCGCCAGGCCCCGGGAAACCACGG |
| Internal bar code: | GTTAGAGAAGAGTTATTACCGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 889 |
| LEAP-Seq percent confirming: | 66.6667 |
| LEAP-Seq n confirming: | 8 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCACTGGAGGCTAGGTGTTG |
| Suggested primer 2: | ATCCCTTTCAAGCCTCCAGC |