Insertion junction: CLIP2.033476_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.033476
Chromosome:chromosome 12
Location:3226827
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre12.g499750 (1 of 89) PF00076 - RNA recognition motif. (a.k.a. RRM, RBD, or RNP domain) (RRM_1) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGCACCAACCGCGTTCTGCTGCACGCCTGCCAGCGCTGCGGCGGCTAGCG
Internal bar code:GTGATATCTAGACTTGCTTCGA

Confirmation - LEAP-Seq

LEAP-Seq distance:4972
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:38
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:38

Suggested primers for confirmation by PCR

Suggested primer 1:GTCGCTCCCTGTCAGACATC
Suggested primer 2:CGGATGGGAAGAACACGACA