| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | - |
| Strain: | CLIP2.033479 |
| Chromosome: | chromosome 12 |
| Location: | 8536568 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g549200 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCCATGGCCCCGGCTCCGCTACTCGTGCCACCCCGCTATCATCACACACT |
| Internal bar code: | TCTGGTGTAAGATACGTGAATA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2233 |
| LEAP-Seq percent confirming: | 97.0588 |
| LEAP-Seq n confirming: | 33 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 34 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GATGGAGTGGGAGTGGTTGG |
| Suggested primer 2: | CCTTACCCAGGCCTTGTCTG |