Insertion junction: CLIP2.033520_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.033520
Chromosome:chromosome 10
Location:1053920
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre10.g425100 (1 of 1) PTHR24185:SF1 - CALCIUM-INDEPENDENT PHOSPHOLIPASE A2-GAMMA intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TAGTGTCGCGTATTGTTTCTGCTCTTCAACACGCACACACACATGCACAC
Internal bar code:GCCACATATTTTAACTGCAGTG

Confirmation - LEAP-Seq

LEAP-Seq distance:1181
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:2
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:GTGGGGACGTCATCATCAGG
Suggested primer 2:AGGTTCCGAAGCAAGTCTGG