| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.033522 |
| Chromosome: | chromosome 7 |
| Location: | 5883355 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g353850 | (1 of 1) K15289 - solute carrier family 35, member F5 (SLC35F5) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGGTAAAGGATGTGCCGGGAGGTAGGTGCGGTAGGCAGCCATGTGCGCC |
| Internal bar code: | AAGGATGGTTGCATTGATCATC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 497 |
| LEAP-Seq percent confirming: | 93.1034 |
| LEAP-Seq n confirming: | 27 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 29 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGTGTGTGAGCTTGACCTGG |
| Suggested primer 2: | AGGTCTGGATTCTGGGTGGT |