Insertion junction: CLIP2.033542_2

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.033542
Chromosome:chromosome 2
Location:7430485
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre09.g392282 DHC2 Axonemal Dynein Heavy Chain 2; (1 of 1) PTHR10676:SF137 - DYNEIN HEAVY CHAIN 1, AXONEMAL CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACTGCTGCGGGAAGTCCGCGCCACCCTCCACCGGCACCGTGCCGCGCTCT
Internal bar code:CGCGGTGGTATTTGGGACGTCG

Confirmation - LEAP-Seq

LEAP-Seq distance:2864
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:3
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:GGACAAGCTGATGGACACCA
Suggested primer 2:CGAACAGCGCCATGATCTTG