Insertion junction: CLIP2.033546_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.033546
Chromosome:chromosome 2
Location:517551
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre02.g076650 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTATGGGCCCCTGCCATGACTGCGTGTGCGAACTCACCCAAATGCGGATC
Internal bar code:GAATTCGTGAGGAATTTAGAGC

Confirmation - LEAP-Seq

LEAP-Seq distance:1302
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:4
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:TCGAACACGCAGACTCGAAA
Suggested primer 2:CCCTCCCCACATCATATGCC