Insertion junction: CLIP2.033570_1

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:+
Strain:CLIP2.033570
Chromosome:plastome
Location:30824
Confidence (%):80
Locus systematic id Locus common name Defline Feature
CreCp.g802279 rps8,2716952 30S ribosomal protein S8; (1 of 1) PTHR11758//PTHR11758:SF17 - 40S RIBOSOMAL PROTEIN S15A // SUBFAMILY NOT NAMED CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CATTAGTAAACCAGGTTTACGCATTTATTCAAACCATAAAGATATTTTAC
Internal bar code:TGGGTACTGCGTGTGAGCGGAT

Confirmation - LEAP-Seq

LEAP-Seq distance:56
LEAP-Seq percent confirming:5.55556
LEAP-Seq n confirming:1
LEAP-Seq n nonconfirming:17
LEAP-Seq n unique pos:18

Suggested primers for confirmation by PCR

Suggested primer 1:TCTTTTGCAGTCGCCTTCCA
Suggested primer 2:TGCAGGAGTTTAACGAGCGT