| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.033606 |
| Chromosome: | chromosome 6 |
| Location: | 3550678 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g278124 | (1 of 1) PTHR12894//PTHR12894:SF10 - CNH DOMAIN CONTAINING // VAM6/VPS39-LIKE PROTEIN | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGACAGATGGAGCACGCCCGCTCGCCCGTCACCAGCACGCGCTGCTGCCG |
| Internal bar code: | GATATGATCGTGTTCCTCCAGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1340 |
| LEAP-Seq percent confirming: | 68.0 |
| LEAP-Seq n confirming: | 17 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 25 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTGGGGAAGGGGAGAGGTAG |
| Suggested primer 2: | CGAACTCGCCAGCTAGCATA |