| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.033608 |
| Chromosome: | chromosome 5 |
| Location: | 2035261 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g236500 | (1 of 1) 3.5.1.23 - Ceramidase / Acylsphingosine deacylase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAGCCCATCATGCTGGACGCGCAGCAGCAGCAGCAGCAGCAGCAGCAGCA |
| Internal bar code: | TCCTGGATCGTTCTCGGCCGTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2315 |
| LEAP-Seq percent confirming: | 96.5517 |
| LEAP-Seq n confirming: | 28 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 29 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACCCCTGCAGCACAAGATA |
| Suggested primer 2: | TGTGATTTTGGGCCCAAGGT |