Insertion junction: CLIP2.033621_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:+
Strain:CLIP2.033621
Chromosome:chromosome 5
Location:1183814
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre05.g248650 (1 of 587) 2.7.11.1 - Non-specific serine/threonine protein kinase / Threonine-specific protein kinase CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTCGCAGCGGGCACCAGCGGTGCGACGGCGCTCCAAAGCGGCACCCGCGA
Internal bar code:ACGACCTAGGTTAAGGTAGGCC

Confirmation - LEAP-Seq

LEAP-Seq distance:3321
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:20
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:20

Suggested primers for confirmation by PCR

Suggested primer 1:ACACCGTCTTCTGCTACTGC
Suggested primer 2:ATACACACACCCAGACGCTG