Insertion junction: CLIP2.033627_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.033627
Chromosome:chromosome 17
Location:4032535
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre17.g728450 (1 of 1) IPR006626//IPR010308//IPR011050 - Parallel beta-helix repeat // TRP-like family // Pectin lyase fold/virulence factor intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCATGTGGGCCCACATGAGGTCGTCTGTGGGGACAGGTGCTGCTGCTGCT
Internal bar code:TCGGTTCGGACCACGGCTCTAC

Confirmation - LEAP-Seq

LEAP-Seq distance:1736
LEAP-Seq percent confirming:94.8718
LEAP-Seq n confirming:37
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:39

Suggested primers for confirmation by PCR

Suggested primer 1:TGTTGTTGGCGAATGTGCTG
Suggested primer 2:CCGCGTTTTCCTTTTCGTGT