| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.033665 |
| Chromosome: | chromosome 9 |
| Location: | 6648349 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g416450 | (1 of 14) PF04577 - Protein of unknown function (DUF563) (DUF563) | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGAATCCCCTGTCCGCTGGCTGGCCTTTCGCTGGGGGTGTCGGCAGGTG |
| Internal bar code: | CCAGAAGAGATGTGATCGACAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3620 |
| LEAP-Seq percent confirming: | 85.2273 |
| LEAP-Seq n confirming: | 75 |
| LEAP-Seq n nonconfirming: | 13 |
| LEAP-Seq n unique pos: | 88 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCGTCAGTGAGTGAAGTGGA |
| Suggested primer 2: | CGACGTACCATAGGCCTGAC |