Insertion junction: CLIP2.033679_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:+
Strain:CLIP2.033679
Chromosome:chromosome 1
Location:4213123
Confidence (%):63
Locus systematic id Locus common name Defline Feature
Cre01.g028423 (1 of 1) 4.1.1.25 - Tyrosine decarboxylase / L-tyrosine carboxy-lyase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCGATGCGGATTCGCCACGCCCCAGGCTCCACCCTTCCCGCGCGCGACCC
Internal bar code:AGACAACGCGCAAGCACAGGGG

Confirmation - LEAP-Seq

LEAP-Seq distance:854
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:1
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:AAACCAACGAAAACGAGCGG
Suggested primer 2:ACACCGCACAGGATGAATGT