| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.033699 |
| Chromosome: | chromosome 13 |
| Location: | 2746216 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g582150 | (1 of 58) PTHR12393//PTHR12393:SF6 - SPHINGOMYELIN PHOSPHODIESTERASE RELATED // SPHINGOMYELIN PHOSPHODIESTERASE 2 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGACTGTATACAAGCGTCGGGCGGCGATGGTGGGCCTTGCGTGGTGGCGC |
| Internal bar code: | GAACGTACTTTTTAAATCCCTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1302 |
| LEAP-Seq percent confirming: | 96.2963 |
| LEAP-Seq n confirming: | 26 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 27 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTAGGGCCAAGCATCCAAGG |
| Suggested primer 2: | ACAACCCTTACGTACTGCGG |