| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.033705 |
| Chromosome: | chromosome 6 |
| Location: | 1599761 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g261400 | DTH1 | Delayed in Triacylglycerol Hydrolysis 1 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GACCTGGGCCTGGACCTCTTCGGTGGCGCGGGAAGGTGAGACCCCTGCTG |
| Internal bar code: | GGGGACTCCAAGAAGCGGTCGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1474 |
| LEAP-Seq percent confirming: | 5.0 |
| LEAP-Seq n confirming: | 3 |
| LEAP-Seq n nonconfirming: | 57 |
| LEAP-Seq n unique pos: | 60 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTCAGAGAACCACCTTGCGA |
| Suggested primer 2: | ACTTTACCGCGCCACCATTA |