| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.033706 |
| Chromosome: | chromosome 1 |
| Location: | 2847257 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g017650 | FAP34,RIB30 | Ribbon binding 30 kDa microtuble inner protein | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TACGGATGCGGCAGCAGGGCGGGCGGCTCACGACATGCGGAAAAGCGAGC |
| Internal bar code: | TATGGAGCTCGGGGTGCAAGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3620 |
| LEAP-Seq percent confirming: | 98.75 |
| LEAP-Seq n confirming: | 79 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 80 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCACACAAGAAAGCCAAGGG |
| Suggested primer 2: | TCGCCTGCATCTCACCTTTT |