| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.033709 |
| Chromosome: | chromosome 12 |
| Location: | 9073354 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g544450 | CSE20 | Predicted protein of CSE family | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACCTGGCTCACCGTGCGCGTCGGCAAGGTGCGCTCCGGCATCATGGCCCC |
| Internal bar code: | GATGACCGGATACCTCTGAGAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4775 |
| LEAP-Seq percent confirming: | 96.2264 |
| LEAP-Seq n confirming: | 51 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 53 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACGCCATAACACTGTGACGT |
| Suggested primer 2: | TGCGTGTACTTGCCGTAACT |