Insertion junction: CLIP2.033742_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.033742
Chromosome:chromosome 13
Location:1862894
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre13.g575366 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGCCTCCCTCAGCTCCCACATCTCCGCCTCCTCCGCGCGCTTGTTGGCGG
Internal bar code:TGTTAGATCGGATGCTCGATGA

Confirmation - LEAP-Seq

LEAP-Seq distance:4798
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:80
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:80

Suggested primers for confirmation by PCR

Suggested primer 1:CCTGGAACCATGTCGAAGCT
Suggested primer 2:GTCGTGCTACTGCTGCTAGT