Insertion cassette: | CIB2 |
Side of cassette: | 5' |
Strand: | + |
Strain: | CLIP2.033746 |
Chromosome: | chromosome 9 |
Location: | 69025 |
Confidence (%): | 80 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre09.g386167 | (1 of 228) IPR029063 - S-adenosyl-L-methionine-dependent methyltransferase | intron |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTTCGCACGAAGCGTGGCTCGGGCACTCCCCCATGAGTCGAAGCTAAGCC |
Internal bar code: | TATAGGTAATCTGTTCCGGCGG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 3321 |
LEAP-Seq percent confirming: | 91.5493 |
LEAP-Seq n confirming: | 65 |
LEAP-Seq n nonconfirming: | 6 |
LEAP-Seq n unique pos: | 71 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GTATGAAGTTGCGAGCGCTG |
Suggested primer 2: | CGCCCCAGTGATTTAATGCG |