| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.033763 |
| Chromosome: | chromosome 1 |
| Location: | 5634896 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g040300 | DNJ9 | Catechol O-methyltransferase; (1 of 1) 2.1.1.6 - Catechol O-methyltransferase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTTGACATGATTGCATGCCTGGGATGCGTGGATGGCGGCGGGTGGCACTG |
| Internal bar code: | CCTGAATTGACTTTGGGGGGAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1345 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 12 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCTCAGACTGTTGCAGCCTG |
| Suggested primer 2: | TGGCACTGACTCAGCAGAAG |