Insertion junction: CLIP2.033770_2

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.033770
Chromosome:chromosome 4
Location:3763347
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre04.g229494 (1 of 70) 3.1.3.16 - Protein-serine/threonine phosphatase / Serine/threonine specific protein phosphatase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTGTCGGAAAACTCGGCACGCCTCCTTCACAGTTTGGCCCCCCCCCTCCT
Internal bar code:CATCGCTGGAGTGTCGGGATGA

Confirmation - LEAP-Seq

LEAP-Seq distance:185
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:2
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:CACCCTGTCGCCATTGTTTG
Suggested primer 2:CCCTTGTCAGCAGGTAAGCA