| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.033778 |
| Chromosome: | chromosome 12 |
| Location: | 9833556 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g546452 | GT90F45,GT90-45 | (1 of 52) PF05686 - Glycosyl transferase family 90 (Glyco_transf_90); GT90 family protein 45 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCTACATCAACGTAAAGGAGGAAGATCCCTCCCCGCCGCCCACCCCGCCA |
| Internal bar code: | GGGTTTATGTGGTACAATTTTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2870 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 61 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 61 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACGTCCGCACTGAAAGATGT |
| Suggested primer 2: | TTGTACGCACGTGCCAAATG |