Insertion junction: CLIP2.033780_2

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:+
Strain:CLIP2.033780
Chromosome:chromosome 1
Location:4230258
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre01.g028600 SDR2 (1 of 1) IPR002347//IPR003560//IPR013968 - Glucose/ribitol dehydrogenase // 2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase // Polyketide synthase, ketoreductase domain; Short-chain dehydrogenase/reductase CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCTTGGGCGCGTACGTGGCGGCGGCGCTCAGCGCCAGGCCTGCGTTCAGC
Internal bar code:GACCAGGTGTACCGCTTGCGCG

Confirmation - LEAP-Seq

LEAP-Seq distance:2101
LEAP-Seq percent confirming:97.7273
LEAP-Seq n confirming:43
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:44

Suggested primers for confirmation by PCR

Suggested primer 1:GGAGACCTACGGCAAGATCG
Suggested primer 2:TGCTCGCCATGTACATGTGT