| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.033783 |
| Chromosome: | chromosome 5 |
| Location: | 1222741 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g800528 | (1 of 1) K11251//K13849 - histone H2A (H2A) // reticulocyte-binding protein (RBP) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGCGCGAAGCGGGGCAGCAGCCGTCGCACCCCAACTGCCTTGCCCCACTG |
| Internal bar code: | TTCGGGCAAGTCCAGATATCAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1012 |
| LEAP-Seq percent confirming: | 2.27273 |
| LEAP-Seq n confirming: | 1 |
| LEAP-Seq n nonconfirming: | 43 |
| LEAP-Seq n unique pos: | 44 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGCAAGAGGAGGAGGAGGAG |
| Suggested primer 2: | GGGTCACACATGAAGGAGGG |