Insertion junction: CLIP2.033793_2

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.033793
Chromosome:chromosome 17
Location:3280288
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre17.g722000 MFT30,SOC1 Major facilitator superfamily transporter; (1 of 1) K08214 - MFS transporter, OCT family, solute carrier family 22 (organic cation transporter), member 18 (SLC22A18) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGGACCGCCACCGTGGGCTCGGCAGTGAGCCTGGTCATGATCCTGGCGCT
Internal bar code:GGCCGCTGGTATATGAATTGTC

Confirmation - LEAP-Seq

LEAP-Seq distance:1292
LEAP-Seq percent confirming:94.0
LEAP-Seq n confirming:47
LEAP-Seq n nonconfirming:3
LEAP-Seq n unique pos:50

Suggested primers for confirmation by PCR

Suggested primer 1:TTTTGTGCCGCGTCAAGAAG
Suggested primer 2:AGCGCTATCCAGTTCATCGG