| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.033810 |
| Chromosome: | chromosome 6 |
| Location: | 5516155 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g286600 | (1 of 1) PF13898 - Domain of unknown function (DUF4205) (DUF4205) | CDS | |
| Cre06.g286650 | DMT4,DMC4 | (1 of 4) K00558 - DNA (cytosine-5)-methyltransferase 1 (DNMT1, dcm); Putative DNA methylase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CATCAAGCTGTCTGTGTCCAAGGACCCGCGAGGCGGCTGGACTGCCAAGA |
| Internal bar code: | TAGCAGGAGCGTATTGTACATG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1002 |
| LEAP-Seq percent confirming: | 35.4839 |
| LEAP-Seq n confirming: | 11 |
| LEAP-Seq n nonconfirming: | 20 |
| LEAP-Seq n unique pos: | 31 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTCTTCTCCGGCAAGGTCAA |
| Suggested primer 2: | GTTTGACGGCAACAAGGACC |