Insertion junction: CLIP2.033824_2

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.033824
Chromosome:chromosome 16
Location:5215132
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre16.g684600 SEC5 (1 of 1) K17637 - exocyst complex component 2 (EXOC2, SEC5); Component of the Exocyst Complex intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGTACTAGCCTATATTTAAGAACGCTTGTGTAAGGTACAATCTATTGATA
Internal bar code:GGGACGGTGTCATCCTGACTAG

Confirmation - LEAP-Seq

LEAP-Seq distance:3974
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:44
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:44

Suggested primers for confirmation by PCR

Suggested primer 1:CAGTTGCATGCCCACCTCTA
Suggested primer 2:ACAAACTACCTACCTGCCGC