Insertion junction: CLIP2.033828_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.033828
Chromosome:chromosome 10
Location:6381772
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre10.g464264 (1 of 1) PTHR13355:SF12 - GCN5-RELATED N-ACETYLTRANSFERASE (GNAT) FAMILY PROTEIN intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCCCCACCATACGTCCCATCATCCACAACCCACCATTCCCCATGTACGCC
Internal bar code:TTTGATTTTCTTAGGGAGGGAT

Confirmation - LEAP-Seq

LEAP-Seq distance:930
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:5
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:5

Suggested primers for confirmation by PCR

Suggested primer 1:TAGCTCCAATTTCGTGGCGT
Suggested primer 2:CGCGTTACATTGCTGCCTAC