Insertion junction: CLIP2.033835_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.033835
Chromosome:chromosome 3
Location:1408238
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre03.g150800 (1 of 45) IPR029052 - Metallo-dependent phosphatase-like CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCGGCGCTGCTACTGCCGGCGCTGCCAACCTTGCTGATCAACCGCCGAAC
Internal bar code:GCTCGTCTTTTAACAATCGATT

Confirmation - LEAP-Seq

LEAP-Seq distance:1430
LEAP-Seq percent confirming:92.8571
LEAP-Seq n confirming:26
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:28

Suggested primers for confirmation by PCR

Suggested primer 1:AGTCACGTCACGTCATGCTT
Suggested primer 2:TGTGTAGGTACGAGGGGTGT