| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.033843 |
| Chromosome: | chromosome 17 |
| Location: | 5257781 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g802106 | (1 of 32) IPR029062 - Class I glutamine amidotransferase-like | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGGAAGTCACGGTACGACAACCGAAGACAACGAGCTGCATACCAGTGAC |
| Internal bar code: | GAGCTCCTTTACTAACAGGGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2443 |
| LEAP-Seq percent confirming: | 83.871 |
| LEAP-Seq n confirming: | 26 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 31 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCGTTTAAGCACGCCTTCCT |
| Suggested primer 2: | AAGCGGTTGAGGTCGGTTAG |