Insertion junction: CLIP2.033856_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.033856
Chromosome:chromosome 6
Location:8386236
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre06.g307850 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CACACACACACACACACACACACACACACACACACACACACACACACACA
Internal bar code:CCATACGGGTACGGTCTCGCAG

Confirmation - LEAP-Seq

LEAP-Seq distance:699
LEAP-Seq percent confirming:55.5556
LEAP-Seq n confirming:5
LEAP-Seq n nonconfirming:4
LEAP-Seq n unique pos:9

Suggested primers for confirmation by PCR

Suggested primer 1:GGGAGGCGTGATCGTTAGAG
Suggested primer 2:AAGGGTCAGTGATGAGCGTG